LCM FISH™ (morphometric analysis)
Highlights:
- Morphometric image analysis was demonstrated on capillary whole blood samples from upper arm collections that underwent capillary fluorescent in situ hybridization (cFISH/FISH).
- Targets included, but not limited to, the following:
- +12, 13q, 17p, 11q
- BCL 1, BCL 2, BCL 6
- +8, -5q, -7q, 20q-
- 13q14, t(11;14) & +11, 17p13, t(4;14),1q21, t(14;16)
- MYC-BA 8q24, BCL-2 t(14;18), BCL-6
- BCL1 t(11;14), P53, FGFR3/IGH t(4;14), 13q14, 1q21, IGH/MAF
- -7/7q-, -5/5q-, 20q12, +8
- 17P13, TRISOMY 12, 13Q14.3, 11Q22.3
- Successful DAPI staining of viable nuclei from capillary blood samples was demonstrated.
- Successful hybridization of >1000 cells (up to 20,000 cells in a single drop of capillary blood) was demonstrated on standardized microscope slide workflows (automated and manual with/without morphometric analysis).
- Morphometric and automated analysis of up to 21 focal planes using digital microscopy was demonstrated to analyze multiple FISH signals.
- Less than 1 minute of analysis time was observed via automated analysis.
- Diploid and normal states were observed in disease-free reference samples, whereby deviations from diploid were observed in abnormal/disease samples.
- Clonal heterogeneity was observed in complex disease samples.
- Clonal tracking and evolution was observed via longitudinal assessments.
- cFISH was paired with TimepointDx LCM™ for additional orthogonal confirmation and molecular insights, all of which was conducted on the same single collected sample and tracked longitudinally over multiple monthly timepoints.
- Images, both raw and synthetic, demonstrated successful monitoring applications, suggesting translational applications in various leukemias and blood-related disorders.
See White Paper for Details.